Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus.
Identifieur interne : 002522 ( Main/Exploration ); précédent : 002521; suivant : 002523Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus.
Auteurs : Yu-Xuan Hou [République populaire de Chine] ; Cheng Peng ; Zheng-Gang Han ; Peng Zhou ; Ji-Guo Chen ; Zheng-Li ShiSource :
- Virologica Sinica [ 1995-820X ] ; 2010.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Agranulocytes (immunologie), Animaux, Anticorps antiviraux (sang), Anticorps neutralisants, Chiroptera (virologie), Expression des gènes, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Interféron gamma (métabolisme), Interleukine-6 (métabolisme), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Réactions croisées, Souris, Souris de lignée BALB C, Tests de neutralisation, VIH (Virus de l'Immunodéficience Humaine) (génétique), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- administration et posologie : Vaccins antiviraux.
- génétique : Adenoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, VIH (Virus de l'Immunodéficience Humaine), Virus du SRAS.
- immunologie : Agranulocytes, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins antiviraux, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- métabolisme : Interféron gamma, Interleukine-6.
- sang : Anticorps antiviraux.
- virologie : Chiroptera.
- Animaux, Anticorps neutralisants, Expression des gènes, Femelle, Glycoprotéine de spicule des coronavirus, Humains, Réactions croisées, Souris, Souris de lignée BALB C, Tests de neutralisation, Vecteurs génétiques.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Animals, Antibodies, Neutralizing, Antibodies, Viral (blood), Chiroptera (virology), Cross Reactions, Female, Gene Expression, Genetic Vectors, HIV (genetics), Humans, Interferon-gamma (metabolism), Interleukin-6 (metabolism), Leukocytes, Mononuclear (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Neutralization Tests, Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , metabolism : Interferon-gamma, Interleukin-6.
- chemical : Antibodies, Neutralizing, Spike Glycoprotein, Coronavirus.
- genetics : Adenoviridae, HIV, SARS Virus.
- immunology : Leukocytes, Mononuclear, SARS Virus.
- isolation & purification : SARS Virus.
- virology : Chiroptera.
- Animals, Cross Reactions, Female, Gene Expression, Genetic Vectors, Humans, Mice, Mice, Inbred BALB C, Neutralization Tests.
Abstract
A group of SARS-like coronaviruses (SL-CoV) have been identified in horseshoe bats. Despite SL-CoVs and SARS-CoV share identical genome structure and high-level sequence similarity, SL-CoV does not bind to the same cellular receptor as for SARS-CoV and the N-terminus of the S proteins only share 64% amino acid identity, suggesting there are fundamental differences between these two groups of coronaviruses. To gain insight into the basis of this difference, we established a recombinant adenovirus system expressing the S protein from SL-CoV (rAd-Rp3-S) to investigate its immune characterization. Our results showed that immunized mice generated strong humoral immune responses against the SL-CoV S protein. Moreover, a strong cellular immune response demonstrated by elevated IFN-γ and IL-6 levels was also observed in these mice. However, the induced antibody from these mice had weaker cross-reaction with the SARS-CoV S protein, and did not neutralize HIV pseudotyped with SARS-CoV S protein. These results demonstrated that the immunogenicity of the SL-CoV S protein is distinct from that of SARS-CoV, which may cause the immunological differences between human SARS-CoV and bat SL-CoV. Furthermore, the recombinant virus could serve as a potential vaccine candidate against bat SL-CoV infection.
DOI: 10.1007/s12250-010-3096-2
PubMed: 20960282
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001605
- to stream PubMed, to step Curation: 001605
- to stream PubMed, to step Checkpoint: 001652
- to stream Ncbi, to step Merge: 002236
- to stream Ncbi, to step Curation: 002236
- to stream Ncbi, to step Checkpoint: 002236
- to stream Main, to step Merge: 002556
- to stream Main, to step Curation: 002522
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus.</title>
<author><name sortKey="Hou, Yu Xuan" sort="Hou, Yu Xuan" uniqKey="Hou Y" first="Yu-Xuan" last="Hou">Yu-Xuan Hou</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name>
</author>
<author><name sortKey="Han, Zheng Gang" sort="Han, Zheng Gang" uniqKey="Han Z" first="Zheng-Gang" last="Han">Zheng-Gang Han</name>
</author>
<author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
</author>
<author><name sortKey="Chen, Ji Guo" sort="Chen, Ji Guo" uniqKey="Chen J" first="Ji-Guo" last="Chen">Ji-Guo Chen</name>
</author>
<author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20960282</idno>
<idno type="pmid">20960282</idno>
<idno type="doi">10.1007/s12250-010-3096-2</idno>
<idno type="wicri:Area/PubMed/Corpus">001605</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001605</idno>
<idno type="wicri:Area/PubMed/Curation">001605</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001605</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001652</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001652</idno>
<idno type="wicri:Area/Ncbi/Merge">002236</idno>
<idno type="wicri:Area/Ncbi/Curation">002236</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002236</idno>
<idno type="wicri:Area/Main/Merge">002556</idno>
<idno type="wicri:Area/Main/Curation">002522</idno>
<idno type="wicri:Area/Main/Exploration">002522</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus.</title>
<author><name sortKey="Hou, Yu Xuan" sort="Hou, Yu Xuan" uniqKey="Hou Y" first="Yu-Xuan" last="Hou">Yu-Xuan Hou</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name>
</author>
<author><name sortKey="Han, Zheng Gang" sort="Han, Zheng Gang" uniqKey="Han Z" first="Zheng-Gang" last="Han">Zheng-Gang Han</name>
</author>
<author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
</author>
<author><name sortKey="Chen, Ji Guo" sort="Chen, Ji Guo" uniqKey="Chen J" first="Ji-Guo" last="Chen">Ji-Guo Chen</name>
</author>
<author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name>
</author>
</analytic>
<series><title level="j">Virologica Sinica</title>
<idno type="eISSN">1995-820X</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term>
<term>Animals</term>
<term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral (blood)</term>
<term>Chiroptera (virology)</term>
<term>Cross Reactions</term>
<term>Female</term>
<term>Gene Expression</term>
<term>Genetic Vectors</term>
<term>HIV (genetics)</term>
<term>Humans</term>
<term>Interferon-gamma (metabolism)</term>
<term>Interleukin-6 (metabolism)</term>
<term>Leukocytes, Mononuclear (immunology)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Neutralization Tests</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (génétique)</term>
<term>Agranulocytes (immunologie)</term>
<term>Animaux</term>
<term>Anticorps antiviraux (sang)</term>
<term>Anticorps neutralisants</term>
<term>Chiroptera (virologie)</term>
<term>Expression des gènes</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Humains</term>
<term>Interféron gamma (métabolisme)</term>
<term>Interleukine-6 (métabolisme)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Réactions croisées</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Tests de neutralisation</term>
<term>VIH (Virus de l'Immunodéficience Humaine) (génétique)</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vecteurs génétiques</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term>
<term>Interleukin-6</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term>
<term>HIV</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adenoviridae</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>VIH (Virus de l'Immunodéficience Humaine)</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Agranulocytes</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Vaccins antiviraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Leukocytes, Mononuclear</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term>
<term>Interleukine-6</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cross Reactions</term>
<term>Female</term>
<term>Gene Expression</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Neutralization Tests</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps neutralisants</term>
<term>Expression des gènes</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Réactions croisées</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Tests de neutralisation</term>
<term>Vecteurs génétiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A group of SARS-like coronaviruses (SL-CoV) have been identified in horseshoe bats. Despite SL-CoVs and SARS-CoV share identical genome structure and high-level sequence similarity, SL-CoV does not bind to the same cellular receptor as for SARS-CoV and the N-terminus of the S proteins only share 64% amino acid identity, suggesting there are fundamental differences between these two groups of coronaviruses. To gain insight into the basis of this difference, we established a recombinant adenovirus system expressing the S protein from SL-CoV (rAd-Rp3-S) to investigate its immune characterization. Our results showed that immunized mice generated strong humoral immune responses against the SL-CoV S protein. Moreover, a strong cellular immune response demonstrated by elevated IFN-γ and IL-6 levels was also observed in these mice. However, the induced antibody from these mice had weaker cross-reaction with the SARS-CoV S protein, and did not neutralize HIV pseudotyped with SARS-CoV S protein. These results demonstrated that the immunogenicity of the SL-CoV S protein is distinct from that of SARS-CoV, which may cause the immunological differences between human SARS-CoV and bat SL-CoV. Furthermore, the recombinant virus could serve as a potential vaccine candidate against bat SL-CoV infection.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Hubei</li>
</region>
<settlement><li>Wuhan</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Chen, Ji Guo" sort="Chen, Ji Guo" uniqKey="Chen J" first="Ji-Guo" last="Chen">Ji-Guo Chen</name>
<name sortKey="Han, Zheng Gang" sort="Han, Zheng Gang" uniqKey="Han Z" first="Zheng-Gang" last="Han">Zheng-Gang Han</name>
<name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name>
<name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name>
<name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
</noCountry>
<country name="République populaire de Chine"><region name="Hubei"><name sortKey="Hou, Yu Xuan" sort="Hou, Yu Xuan" uniqKey="Hou Y" first="Yu-Xuan" last="Hou">Yu-Xuan Hou</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002522 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002522 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:20960282 |texte= Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20960282" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
This area was generated with Dilib version V0.6.33. |